Suiwen Hou
Lanzhou University
Distinguished Professor of Changjiang Scholars of Ministry of Education and serves as Dean of the College of Life Sciences. His research interests focus on stomatal development, especially in gramineal crops, and the roles of type one protein of phosphatases (TOPPs) in plant development and physiology. His group identified novel regulators of stomatal development, including early synthetases of plant sterol and RNA Polymerase II complex, the key transcription factors in rice stomatal development, and the FLP in meristemoid cell differentiation. His group also uncovered the mechanism by which TOPPs regulate plant growth, pavement cell morphogenesis, light response, seed germination, immunity and autophagy.
Title of presentation
Clathrin-mediated endocytosis regulates stomatal development in Arabidopsis
Authors
Chi Zhang, Liang Chen, Tianwei Hu, Ping Li, Chao Wang, Jianwei Pan, Suiwen Hou
Key Laboratory of Gene Editing for Breeding, Gansu Province, Key Laboratory of Cell Activities and Stress Adaptations, Ministry of Education, School of Life Sciences, Lanzhou University, Lanzhou, 730000, China
Abstract
Clathrin-mediated endocytosis (CME) is a highly conserved endocytosis pathway in eukaryotic cells and plays a crucial role in the endocytosis of membrane proteins. ERECTA family (ERf) receptor-like kinases are known to receive EPFs signals and transmit them to the downstream MAPK cascade pathway in order to regulate stomatal development. However, whether CME mediates the endocytosis of ERf remains unclear. In this study, we found that CME component mutants show clustering stomatal and adaptor protein AP2σ subunit is able to recognize ERf for the first time. CME acts genetically downstream of EPF and upstream of YODA, and works together with ERf, playing a negative regulatory role in stomatal development. When CME is deficient, the endocytosis and degradation of ERf are inhibited. It is more important that mutation of the motif in the cytoplasmic region of ERECTA that is recognized by AP2σ suppressed the endocytosis of ERECTA. Moreover, the deficiency of CME resulted in a slower transmission of EPF-ERf to MAPK cascade, leading to increased expression of SPCH and thus promotion of stomatal production. Overall, our findings indicate that CME plays a crucial role in the signal transduction of EPF-ERf-YODA by mediating the endocytosis of ERf during stomatal development.
My Sessions
Clathrin-mediated endocytosis regulates stomatal development in Arabidopsis
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Presentation