Yuh-Shuh Wang
University of Tartu
Originated from Taiwan, Yuh-Shuh Wang has been interested in the molecular aspect of plant development. She studied genes involved in sweet potato tuber development for her master thesis in National Taiwan University. She then pursued PhD degree in the field of Forest Molecular Genetics and Biotechnology in Michigan Technological University, studying vascular development in aspen trees. During her postdoc years, she began delving into Arabidopsis research for the vast molecular tools and genetic information available. Currently, she is associate professor in the University of Tartu, and her research focuses on stomata responses to environmental stimuli, in particular to CO2 changes.
Title of presentation
Regulation of CO2-induced stomatal movements via HT1 communication between plasma membrane and chloroplasts
Authors
Yuh-Shuh Wang , Chung-Yueh Yeh, Oleksii Yadlos, Hannes Kollist
Institute of Bioengineering, University of Tartu, Nooruse 1, Tartu 50411, Estonia
Abstract
The intercellular concentration of CO2 , mainly affected by the photosynthetic and (photo)respiratory activities, needs to be sensed by guard cells to orchestrate stomatal movements. We have recently identified a CO2 /HCO3 --sensing mechanism composed of the MITOGEN ACTIVATING PROTEIN KINASE (MPK) 12/4 and the Raf-like kinase HIGH LEAF TEMPERATURE 1 (HT1) at the plasma membrane. Surprisingly, we found that the predominantly plasma membrane-localized HT1 can be associated with chloroplasts when possessing a specific amino acid mutation or a short internal deletion. In addition, upon interacting with a specific splice form of the β-CARBONIC ANHYDRASE 1 (βCA1), the HT1:βCA1 complex is clearly localized to chloroplasts as punctate structures. Furthermore, we tethered HT1 to either plasma membrane or chloroplast by adding respective target signal peptides and a fluorescent protein tag. Transgenic plants expressing a plasma membrane-tethering sequence in the ht1 knockout background responded to CO2 changes normally as the wild-type plants, whereas only partial low CO2 -induced stomatal opening was restored in plants with a chloroplast-targeting signal. We propose that shuttling of the HT1 between plasma membrane and chloroplasts may coordinate CO2 signals derived from different cellular compartments, and may regulate opening and closing responses differently depending on its subcellular localization.
My Sessions
Regulation of CO2-induced stomatal movements via HT1 communication between plasma membrane and chloroplasts
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Presentation