Shouguang Huang
University of Wuerzburg
Shouguang Huang is a habilitation candidate at the University of Wuerzburg and a core member of Prof. Rainer Hedrich's group. He is dedicated to studying the regulation of stomatal movement, particularly focusing on the roles of H+, Ca2+, and electrical signals, using optogenetic and electrophysiological techniques. His studies have shown the potential of optogenetics in unraveling intricate signal pathways and mechanisms in guard cells, while also providing valuable tools for addressing other scientific questions in the field of plant science.
Title of presentation
Guard cells count cytosolic Ca2+ signals to regulate stomatal dynamics
Authors
Shouguang Huang , Rob Roelfsema, Alistair M. Hetherington, Rainer Hedrich
Plant Molecular Biology and Biophysics, University of Wuerzburg, Julius-von-Sachs-Platz 2, 97082, Wuerzburg, Germany
Abstract
Transient increases in the cytosolic Ca2+ concentration of guard cells have been proposed to regulate opening and closure of stomatal pores in the leaf surface of plants. However, the mechanism by which these Ca2+ signatures are generated and translated into stomatal movement is still largely unresolved. We used a Ca 2+-permeable variant of ChannelRhodopsin 2 (ChR2-XXM2.0) that was stimulated with blue light (BL) pulses. Activation of the ChR2-XXM2.0 channel provoked cytosolic Ca2+ sparks that were amplified by a Ca2 2+-induced Ca2+-release (CICR) mechanism, which was linked to the endoplasmic reticulum (ER). Repetitive BL pulses caused series of Ca2+ signals that activated S-type anion channels and determined the speed of stomatal closure. Guard cells thus translate Ca 2+ sparks to optimize leaf transpiration to match the prevailing environmental conditions.
My Sessions
Guard cells count cytosolic Ca2+ signals to regulate stomatal dynamics
-
Presentation