Danilo Daloso
Federal University of Ceara
Danilo M. Daloso graduated in Biological Sciences in the State University of Mato Grosso do Sul, Brazil, and obtained the PhD in Plant Physiology in the Federal University of Viçosa (UFV), Brazil. During the PhD, Danilo also worked at the Max Planck Institute for Molecular Plant Physiology (MPIMP) (Germany), under the supervision of Dr. Alisdair R. Fernie. Dr. Daloso defended his doctoral thesis with the works carried out at UFV and MPIMP. Dr. Daloso worked three years as postdoctoral researcher in UFV and MPIMP. Since 2016, Dr. Daloso is an associate professor in the Department of Biochemistry and Molecular Biology at the Federal University of Ceará, Brazil. Dr. Daloso is the coordinator of the Plant Metabolism Laboratory (LabPlant) and the current president of the Brazilian Society of Plant Physiology. Dr. Daloso works are mainly focus stomatal physiology and guard cell metabolism, but he is also developing works on redox biology and plant stress.
Title of presentation
Disentangling the role of sucrose for stomatal movement regulation
Authors
Francisco Bruno S. Freire, Eva G. Morais, Moaciria S. Lemos, Priscila A. Auler, Werner C. Antunes, Jorge Gago, Danilo M. Daloso
LabPlant, Department of Biochemistry and Molecular Biology, Federal University of Ceará. Fortaleza-CE, 60451-970, Brazil. Department of Biology, State university of Maringá, Maringá-PR, 87020-900, Brazil Research Group on Plant Biology Under Mediterranean Conditions, Agro-Environmental and Water Economics Institute, University of the Balearic Islands, Palma de Mallorca, Spain.
Abstract
The role of sucrose for stomatal movement regulation has long been debated, with several controversial studies and theories. Here we demonstrated that sucrose concentration at the leaf apoplast underpin the diel course of tobacco stomatal conductance (gs), in which the daily stomatal opening and closure were associated with low and high concentration of apoplastic sucrose, respectively. In agreement with this, exogenously applied sucrose increased the speediness of both stomatal opening and closure in a concentration-dependent manner. We further showed that the light-induced stomatal opening is closely associated to the dynamic of sucrose and organic acids within guard cells. Interestingly, these sucrose-mediated stomatal responses were drastically reduced in plants with diminished capacity to import sucrose to their guard cells, highlighting that sucrose importation to these cells is important to modulate the magnitude of both stomatal opening and closure. Modelling analysis highlights that the metabolism of the apoplast rather than the leaf is the major determinant of the daily gs. Our results collectively indicate that sucrose is a master regulator of the daily gs, being capable of inducing and accelerating both stomatal opening and closure in a concentration and location of accumulation dependent manner.
My Sessions
Disentangling the role of sucrose for stomatal movement regulation
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Presentation