Portable Field-Based Stomatal Phenotyping System for Live Plant Leaves

L. He
Huazhong Agricultural University, No.1 Shizishan Street, Hongshan District, Wuhan, 430070, China

Stomata are the main channels for gas exchange between the internal and external environments of plants, affecting the transpiration intensity and photosynthesis of plants. Aiming at the problems of traditional stomatal phenotype measurement methods, such as being destructive and time-consuming, this work takes live corn leaves in the field as the research object, and based on embedded devices, designs a portable stomatal phenotype measurement device using deep learning and an improved level set algorithm. This device can extract the stomatal phenotypes of live leaves in the field in real-time, with a stomatal detection accuracy of over 99% and a pore segmentation coefficient of determination over 0.85. Additionally, a web portal has been established for global users to remotely access and use the device, which can facilitate future research on the dynamics of stomatal phenotypes in large crop populations and the analysis of genetic mechanisms.